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recombinant shhn  (R&D Systems)


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    Structured Review

    R&D Systems recombinant shhn
    Recombinant Shhn, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+shhn/Recombinant+Human+Sonic+Hedgehog%2FShh+(C24II)+N-Terminus/pmc11423920-295-46-48
    Average 94 stars, based on 101 article reviews
    recombinant shhn - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: Ptch2 mediates the Shh response in Ptch1 −/− cells
    Article Snippet: Cell Tracker Green was from Invitrogen. .. Recombinant ShhN was from R&D Systems. .. Immunohistochemistry Antibodies for mouse Pax7 (1:20), HB9 (1:10), Nkx2.2 (745-A5; 1:10) and Shh (5E1; 1:10) were from the Developmental Studies Hybridoma Bank.

    Article Title: A cell-autonomous role for primary cilium-mediated signaling in long-range commissural axon guidance
    Article Snippet: The microfluidic chips were coated with 20 μg/ml poly-L-lysine (Sigma-Aldrich, P-12374) and 20 μg/ml laminin (Invitrogen, 23017-015) following the protocol of the manufacturer (XonaChip TM protocol for primary murine neurons). .. On the day of stimulation (1 or 8 days in vitro ), culture medium was discarded from all wells, and 140 μl commissural neuron medium were added to both left wells (connected to the soma chamber) and 130 μl of the same medium containing 2.5 μg/ml recombinant ShhN-6xHis [R&D Systems, 1845-SH; 1:40 dilution in medium of stock solution solubilized in 0.1% bovine serum albumin (BSA)] were given to both wells connected to the axonal chamber. ..

    Article Title: Hedgehog chemotaxis is mediated by Smoothened located outside the primary cilium
    Article Snippet: Cell Tracker Green CMFDA (5-chloromethylfluorescein diacetate) was from Invitrogen (Carlsbad, CA). .. Recombinant ShhN was from R&D Systems (Minneapolis, MN). ..

    Article Title: Sonic Hedgehog Influences the Balance of Osteogenesis and Adipogenesis in Mouse Adipose-Derived Stromal Cells
    Article Snippet: .. Dulbecco’s modified Eagle’s medium and penicillin– streptomycin were purchased from Gibco Life Technologies, fetal bovine serum from Omega Scientific, recombinant ShhN and recombinant BMP-2 from R and D Systems, and cell culture supplies from Corning, Inc. .. Animals, tissue harvest, and primary cell culture Mouse ASCs were isolated from fifty 3-week-old CD-1 mice (Charles River Laboratories) as previously described.17 For in vivo experiments, ASCs were isolated from CD-1 mice in which the green fluorescent protein (GFP) was linked to a b-actin promoter.

    In Vitro:

    Article Title: A cell-autonomous role for primary cilium-mediated signaling in long-range commissural axon guidance
    Article Snippet: The microfluidic chips were coated with 20 μg/ml poly-L-lysine (Sigma-Aldrich, P-12374) and 20 μg/ml laminin (Invitrogen, 23017-015) following the protocol of the manufacturer (XonaChip TM protocol for primary murine neurons). .. On the day of stimulation (1 or 8 days in vitro ), culture medium was discarded from all wells, and 140 μl commissural neuron medium were added to both left wells (connected to the soma chamber) and 130 μl of the same medium containing 2.5 μg/ml recombinant ShhN-6xHis [R&D Systems, 1845-SH; 1:40 dilution in medium of stock solution solubilized in 0.1% bovine serum albumin (BSA)] were given to both wells connected to the axonal chamber. ..

    Modification:

    Article Title: Sonic Hedgehog Influences the Balance of Osteogenesis and Adipogenesis in Mouse Adipose-Derived Stromal Cells
    Article Snippet: .. Dulbecco’s modified Eagle’s medium and penicillin– streptomycin were purchased from Gibco Life Technologies, fetal bovine serum from Omega Scientific, recombinant ShhN and recombinant BMP-2 from R and D Systems, and cell culture supplies from Corning, Inc. .. Animals, tissue harvest, and primary cell culture Mouse ASCs were isolated from fifty 3-week-old CD-1 mice (Charles River Laboratories) as previously described.17 For in vivo experiments, ASCs were isolated from CD-1 mice in which the green fluorescent protein (GFP) was linked to a b-actin promoter.

    Cell Culture:

    Article Title: Sonic Hedgehog Influences the Balance of Osteogenesis and Adipogenesis in Mouse Adipose-Derived Stromal Cells
    Article Snippet: .. Dulbecco’s modified Eagle’s medium and penicillin– streptomycin were purchased from Gibco Life Technologies, fetal bovine serum from Omega Scientific, recombinant ShhN and recombinant BMP-2 from R and D Systems, and cell culture supplies from Corning, Inc. .. Animals, tissue harvest, and primary cell culture Mouse ASCs were isolated from fifty 3-week-old CD-1 mice (Charles River Laboratories) as previously described.17 For in vivo experiments, ASCs were isolated from CD-1 mice in which the green fluorescent protein (GFP) was linked to a b-actin promoter.



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    Image Search Results


    Journal: eLife

    Article Title: Two-way Dispatched function in Sonic hedgehog shedding and transfer to high-density lipoproteins

    doi: 10.7554/eLife.86920

    Figure Lengend Snippet:

    Article Snippet: Recombinant DNA reagent , ShhN/ C25A/S Shh , Grobe Lab , OriGene NM_009170 , Monolipidated Shh.

    Techniques: Variant Assay, Expressing, Knock-Out, Control, Transfection, Construct, Plasmid Preparation, Recombinant, Functional Assay, Software, Western Blot, Derivative Assay, Binding Assay, Immunoprecipitation